tTMB-P9(matrix) Reference Standard

CBP80001-9

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I. Background

Tumor mutational burden (TMB) shows great promise as a biomarker for immuno-oncology (I-O) therapy response, but progress is hindered by the lack of a standardized TMB measurement by targeted NGS panels, and an absence of TMB reference materials.

Sample ID	Description	Cut off=1%	Cut off=2%	Cut off=3%	Cut off=4%	Cut off=5%
DC208D0421	10%T+90%N	22.94	19.72	16.53	12.50	8.41
DC208D0422	5%T+95%N	15.75	10.50	5.82	2.92	1.37
DC208D0423	2%T+98%N	8.29	2.12	0.75	0.42	0.21
DC208D0424	1%T+99%N	4.15	0.95	0.27	0.18	0.09
500xWES; IDT xGenExome Research Panel v1.0捕獲，Target Region 39M, Probe Region 51M; HiSeq X-TEN，af選擇0.01 cut off，call突變，再過濾，然后在按照梯度cut off計(jì)算TMB。 Tumor sample: stage 4, adenocarcinoma Lung, Female Normal sample: B lymphoblast from the same individual

計(jì)算數(shù)據(jù)下載：

DC208D0421.filter.0.01.tag	DC208D0423.filter.0.01.tag
DC208D0421.filter.0.02.tag	DC208D0423.filter.0.02.tag
DC208D0421.filter.0.03.tag	DC208D0423.filter.0.03.tag
DC208D0421.filter.0.04.tag	DC208D0423.filter.0.04.tag
DC208D0421.filter.0.05.tag	DC208D0423.filter.0.05.tag
DC208D0422.filter.0.01.tag	DC208D0424.filter.0.01.tag
DC208D0422.filter.0.02.tag	DC208D0424.filter.0.02.tag
DC208D0422.filter.0.03.tag	DC208D0424.filter.0.03.tag
DC208D0422.filter.0.04.tag	DC208D0424.filter.0.04.tag
DC208D0422.filter.0.05.tag	DC208D0424.filter.0.05.tag

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tTMB-P9(matrix) Reference Standard