hIL20/IL22/IL24 Triple Effector Reporter Cell
CBP74228
| I. Background | |
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IL白細胞介素 10（IL-10 ）相關(guān)細胞因子家族包括 IL-10、IL-19、IL-20、IL-22、IL-24、IL-26、IL-28A/B 和 IL-29 等幾個成員，它們也屬于 2α類螺旋細胞因子。IL-19、IL-20、IL- 22、IL-24 和 IL-26 也屬于 IL-20 亞家族。IL-20 亞家族是皮膚炎癥的核心驅(qū)動者（尤以銀屑病為典型），同時參與 Th2 疾病（哮喘、AD）及腫瘤微環(huán)境調(diào)控。 |
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| II. Description | |
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hIL20/IL22/IL24 Triple Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi)IL20/IL22/IL24 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
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| III. Introduction | |
| Expressed gene: | IL22R1、IL20R2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data
Figure 2. Recombinant hIL20/IL22/IL24 Triple Effector Reporter Cell stably expressing IL22Rα.
Figure 3. Dose Response of Ligands in hlL20/IL22/lL24 Triple Effector Reporter Cell(C30).
Figure 4. Inhibition of Human lL22-induced Reporter Activity by lL22Ra Blocking Ab or lL22 Neutralization Ab in hIL20/IL22/IL24 Triple Effector Reporter Cell(C30).
Figure 5. Inhibition of Human lL20-induced Reporter Activity by lL20 Neutralization Ab in hlL20/IL22/IL24 Triple Effector Reporter Cell(C30). |
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