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U251-Luc

CBP30207L

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I. Introduction
Host Cell: U251
Expressed gene: Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM+1%Non Essential Amino Acids NEAA+1mM Sodium Pyruvate NaP+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen
 
II. Description of Host Cell Line
Organism: Homo sapiens,human
Tissue: Brain
Disease: Human glioblastoma astrocytoma
Morphology: Pleomorphic/astrocytoid
Growth Properties: Adherent
 
III. Representative Data
CBP30207L 信號(hào)

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 508±6(Mean±SD) on U251 cells while 49992742 ±1810197(Mean±SD) on U251-Luc cells.

 
IV. Stablity Test
CBP30207L 穩(wěn)定性

Figure. Stablity test results. U251-Luc cells showed at least 32 passage stability with similar luminescence unit.


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