GCGR/CRE-Luc/HEK293
CBP71345
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產(chǎn)品描述
產(chǎn)品數據庫
| I. Background | |
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胰高血糖素是一種胰腺肽激素，作為胰島素的反調節激素，刺激葡萄糖通過(guò)肝臟釋放， 維持葡萄糖穩態(tài)。GCGR 是一種 B 類(lèi) GPCR，最初被描述為與腺苷酸環(huán)化酶功能相連的胰 高血糖素結合體。它在維持葡萄糖穩態(tài)中起關(guān)鍵作用，因此被認為是治療糖尿病的一個(gè)有 價(jià)值的靶點(diǎn)。 |
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| II. Description | |
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GCGR CRE-Luc HEK293 報告基因藥靶模型很好的模擬了體內 GCGR 的信號轉導過(guò)程， 原理見(jiàn)下圖所示。
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| III. Introduction | |
| Host Cell: |
HEK293 |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM +10%FBS+2μg/ml puromycin+200μg/ml hygromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GCGR |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: |
Embryonic kidney
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| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 2. Recombinant GCGR/CRE-Luc/HEK293 constitutively expressing GCGR. |
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Figure 3. Dose response of Glucagon(1-29) in GCGR CRE-Luc HEK293(C21). |
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