BRAF K601N/BaF3
CBP73386
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| I. Introduction | |
| Cell Line Name： | BRAF K601N/BaF3 |
| Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Application: | Anti-proliferation assay and PD assay |
| Freeze Medium: | 90% FBS+10% DMSO |
| Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| II. Background | |
| BRAF K601N lies within the protein kinase domain of the Braf protein (UniProt.org). K601N confers a gain of function on Braf, as indicated by increased phosphorylation of MEK and ERK in cell in culture and induction of cell proliferation and cell viability in culture. | |
| III. Representative Data | |
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1. WB of BRAF K601N/BaF3
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2. Sanger of BRAF K601N/BaF3
Figure 2. Sanger of BRAF K601N/BaF3 |
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3. Anti-proliferation assay |
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Figure 3. CTG Proliferation Assay of BRAF K6D1N BaF3 Cells (C12). |
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