D341 Med（人腦髓母細胞瘤）
CBP60779
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| I. General information | |
| Synonyms: | D341 Med |
| Background: | The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma. |
| Species: | Homo sapiens, human |
| Tissue: | brain/cerebellum |
| Disease: | medulloblastoma |
| Gender: | male, 3.5 years |
| Morphology: | spheroid |
| Growth Mode: | suspension |
| Doubling Time: | 37 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,13 D16S539: 12,14 D5S818: 11,12 D7S820: 9,13 THO1: 6,9.3 TPOX: 8,11 vWA: 17,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 20% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) D341 Med完全培養基，# CBP60779M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. |
| Cellular Products: | neurofilament proteins |
| Tumor Formation: | Yes, in nude mice; forms serially transplantable intercranial and subcutaneous tumors Yes, in soft agar |
| Comments: | The cells produce tumors in nude mice. The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. The c-myc oncogene is amplified in D341 Med cells. For more information, please contact Cobioer (4008-750-250). |
