NCI-H446（小細胞肺癌）
CBP61025
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| I. General information | |
| Synonyms: | NCI-H446 |
| Background: | The NCI-H446 cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with small cell cancer of the lung. |
| Species: | Homo sapiens, human |
| Tissue: | lung; derived from metastatic site: pleural effusion |
| Disease: | carcinoma; small cell lung cancer |
| Gender: | male, 61 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | mixed, adherent and suspension |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 13,14 D13S317: 8 D16S539: 12 D5S818: 11 D7S820: 10,11 THO1: 8,9.3 TPOX: 9,11 vWA: 18,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H446完全培養基，# CBP61025M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology.) |
| Comments: | The morphology of the original tumor was not characteristic of SCLC. The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase. It does not have detectable levels of L-DOPA decarboxylase, bombesin, vasopressin, oxytocin or gastrin releasing peptide. C-myc DNA sequences are amplified about 20 fold, and there is a 15 fold increase in c-myc RNA relative to normal cells. The line was originally propagated in serum free RPMI 1640 medium supplemented with 10 nM hydrocortisone, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 10 nM 17-beta-estradiol, and 30 nM sodium selenite. The cells form transplantable tumors with non-typical SCLC histology. For more information, please contact Cobioer (4008-750-250). |
