SVCT（人SV40轉化乳腺上皮細胞）
CBP61166
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| I. General information | |
| Synonyms: | SVCT |
| Background: | SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent. |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | Human breast epithelium, SV40 transformed |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 7,11 D5S818: 12,13 (PubMed=28889351) 13(CCRID=5301HUM-KCB15018YJ) D7S820: 8,11 D13S317: 12,14 D16S539: 9,12 D21S11:29,35 TH01: 8,9 (CCRID=5301HUM-KCB15018YJ) 9(PubMed=28889351) TPOX: 8,12 vWA: 16,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
