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CX-1(人結腸癌細胞)

CBP61230

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I. General information 
Cell Name: CX-1
Cell type: Colon adenocarcinoma (derivative of HT-29)
Species: Homo sapiens,human
Description: Established from the tumor tissue of a 44-year-old woman with well-differentiated colon adenocarcinoma in 1984; cell line is a subclone of cell line HT-29 (DSM ACC 299)
Morphology: Small circular cells, adherent, grow to large colonies in which the single cells are no longer discernible
Mycoplasma: Negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: Cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Culture Medium:

DMEM+10%FBS

CX-1完全培養基, #CBP61230M
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Cytogenetics: Human hypertriploid karyotype with 14% polyploidy - 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?) (p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12), add(21)(p11) - two small markers present - carries 3 markers similar to those present in HT-29 - rearrangements of chs 1, 7, 17 typical of colon carcinoma
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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