T84（人結腸癌細胞）
CBP60021
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| I. General information | |
| Synonyms: | T84 |
| Background: | The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice. |
| Species: | Homo sapiens, human |
| Tissue: | colon; derived from metastatic site: lung |
| Disease: | colorectal carcinoma |
| Gender: | male,72 years |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 10 D13S317: 9 D16S539: 10,11 D5S818: 12 D7S820: 8,10 THO1: 6,9 TPOX: 8,11 vWA: 17,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM/F12(1:1) + 10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Receptor Expression: | neurotransmitter, expressed peptide hormone, expressed |
| Genes Expressed: | carcinoembryonic antigen (CEA), 600 ng/mL per 106 cells per 10 days; keratin |
| Cellular Products: | carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin |
| Tumor Formation: | Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments: | The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice. After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport.This line exhibits tight junctions, and desmosomes between adjacent cells. The cells are positive for keratin by immunoperoxidase staining. For more information, please contact Cobioer (4008-750-250). |
